[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"skill:01M6HASARTFBN5KBB3ZTCPQGWX":3,"skill-same:01M6HASARTFBN5KBB3ZTCPQGWX":54},{"skillNo":4,"name":5,"nameZh":6,"description":7,"descriptionZh":6,"repositoryFullName":8,"ownerLogin":9,"ownerAvatarUrl":10,"ownerAccountNo":11,"ownerDisplayName":12,"repositoryNo":13,"starsCount":14,"licenseSpdxId":15,"licenseFileUrl":6,"sourceHtmlUrl":16,"categories":17,"keywords":18,"publishedAt":25,"linkOnly":26,"markdownContent":27,"markdownBody":28,"frontMatter":29,"refName":32,"commitSha":33,"skillDirectory":34,"forksCount":14,"repositoryHtmlUrl":35,"repositoryHomepageUrl":6,"ownerHtmlUrl":36,"ownerBlogUrl":37,"ownerDescription":38,"updatedAt":39,"install":40,"resources":53},"01M6HASARTFBN5KBB3ZTCPQGWX","synthetic-biology",null,"Engineering biological systems","NeuralBlitz\u002FMito","NeuralBlitz","https:\u002F\u002Favatars.githubusercontent.com\u002Fu\u002F79354388?v=4","01M6H8T2TFH477HRS0KTSTKR2E","David 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Key Concepts\n\n### Design Principles\n\n**Standardization**\n- BioBricks: Standardized parts\n- RFC standards: Assembly rules\n- SBOL: Data exchange format\n\n### Genetic Circuits\n\n```python\n# Example: Toggle switch design\nclass ToggleSwitch:\n    \"\"\"\n    Bistable genetic switch.\n    \"\"\"\n    def __init__(self):\n        self.components = {\n            'promoter1': 'Repressor 1',\n            'promoter2': 'Repressor 2',\n            'repressor1': 'Inhibits promoter2',\n            'repressor2': 'Inhibits promoter1'\n        }\n    \n    def states(self):\n        return {\n            'state_A': 'Repressor1 ON, Repressor2 OFF',\n            'state_B': 'Repressor1 OFF, Repressor2 ON'\n        }\n\n# Circuit types\ncircuit_types = {\n    'inverter': 'NOT gate',\n    'amplifier': 'Signal enhancement',\n    'oscillator': 'Repressible gene expression',\n    'memory': 'State persistence',\n    'logic_gates': 'AND, OR, NAND, NOR'\n}\n```\n\n### Metabolic Engineering\n\n- Pathway design\n- Cofactor balancing\n- Flux optimization\n- Enzyme engineering\n- Compartmentalization\n\n### Applications\n\n- Biofuels production\n- Pharmaceutical synthesis\n- Bioremediation\n- Biosensors\n- Therapeutic cells\n- Living materials\n","## What I do\n\n- Design and construct new biological parts\n- Engineer metabolic pathways\n- Create synthetic gene circuits\n- Develop biosensors and therapeutics\n- Program cells with new functions\n- Apply design-build-test-learn cycles\n\n## When to use me\n\n- When engineering new biological functions\n- When designing gene circuits\n- When creating metabolic pathways\n- When developing biosensors\n- When programming cells\n- When building synthetic organisms\n\n## Key Concepts\n\n### Design Principles\n\n**Standardization**\n- BioBricks: Standardized parts\n- RFC standards: Assembly rules\n- SBOL: Data exchange format\n\n### Genetic Circuits\n\n```python\n# Example: Toggle switch design\nclass ToggleSwitch:\n    \"\"\"\n    Bistable 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